方法采用结扎大鼠颈动脉及进入低氧仓的方法建立脑缺血、缺氧损伤模型；

Methods The model of brain ischemic and hypoxic injuries in rats was established by unilateral carotid artery ligature just for 2 h.

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而构建缺氧培养环境作为确立细胞缺氧损伤模型实验的基础工作是极其重要的。

And as the basic for the whole experimental work, designing the model of hypoxia cell culture is very important.

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方法制备心肌组织缺氧损伤模型，观察不同剂量的地高辛抗血清对缺氧损伤心肌组织内源性洋地黄样因子水平和心肌细胞膜atp酶活性的影响。

Methods it was observed that different concentration anti_digoxin antiserum effect on endogenous digitalis_like factor and cell membrane ATPase activity in hypoxic myocardium model.

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