丝线结扎 Silk ligature
All the experimental animals were given silk ligature around the cervix of the teeth and were provided with high sugar diet. With the period, the clinical status were obtained from the experimental teeth, at 1, 2, 4 and 8 weeks.
方法⑴4只成年Beagle犬,选其24颗前牙颈部龈缘水平结扎丝线并饲以高糖饮食,分别于1w、2w、4w、8w检查实验动物的牙周状况,记录结果。
参考来源 - 骨保护素基因修饰的自体骨髓基质细胞在Beagle犬牙周组织再生术中的作用·2,447,543篇论文数据，部分数据来源于NoteExpress
方法：结扎丝线法建立大鼠急性牙髓炎症模型和免疫组织化学技术观察牙髓CGRP - IR神经纤维的变化。
Methods: Rat acute pulpitis model was established by silk thread ligation and the change of CGRP IR nerve fibers was observed with immunohistochemical method.
上下颌牙用丝线结扎法形成局部刺激因素。
The local stimulation was caused by tiring silk thread to teeth.
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