我们收集了库普弗细胞的条件培养基(Kupffer cell conditioned medium，KCCM)，作用于培养的肝细胞，观察其对肝细胞的代谢和增殖变化以及损伤情况，以探讨内毒素对肝细胞的损伤机制。
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目的探索从骨肉瘤细胞条件培养基中提取骨形态蛋白（BMP）的方法，并测定其生物学活性。
Objective: To find out a method of extraction and purification of bone morphogenetic protein (BMP) from osteosarcoma cell conditioned medium, and evaluate the biological activity of BMP.
目的探索从骨肉瘤细胞条件培养基中提取骨形态蛋白(BMP)的方法，并测定其生物学活性。
Objective: To find out a method of extraction and purification of bone morphogenetic protein (BMP) from osteosarcoma cell conditioned medium, and evaluate the biological activity of BMP.
体细胞胚的诱导及其频率高低与材料基因型、培养基和培养条件有关。
The somatic embryo induction and induction frequency were related to genotypes, media and culture conditions.
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