同时也说明细胞水平的抗病性与植株水平的抗病性不完全对应,但这种突变体筛选途径是切实有效的。
These results also showed that the resistance at the cell level were not always as same as whole plants.
方法:1、应用MTT方法在细胞水平上进行中药粗提物的筛选,首先建立人肾脏 近 端 小 管上皮细胞株(HK-2)及卵巢癌细胞株(OC-3)的生长曲线,以确定接种密度及给药时机;
Methods: 1. In vitro, with MTT, the growth curve of HK-2 cells and OC-3 cells had been built in order to decide the density to inoculate and when to administration.
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