对部分经潮霉素筛选得到的再生植株进行了多次重复P CR检测,发现其中40 %以上的潮霉素抗性植株均表现出较强的阳性反应,初步证明几丁质酶基因已整合到油菜细胞核基因组中。
PCR test of the resistant plants indicated that 40% of the Hyg-resistant plants showed strong positive reaction, suggesting that chitinase gene had been integrated into the genome of rapeseed.
创造嵌入式生物体,很长时间以来是通过繁殖技术,最近一段时间,通过将自然基因组植入去除细胞核的靶细胞中来完成。
Chimeric organisms have long been created through breeding and, more recently, through the transfer of native genomes into denucleated target cells.
这些变化很容易被识别,因为线粒体d NA基因组的规模不大- - -仅仅16 000个单位- - -相比之下,细胞核内的基因组却有30亿个单位。
These changes are easy to identify because the mitochondrial DNA genome is so small - just 16, 000 units - compared with the three billion units of the genome in the cell's nucleus.
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