hTERT诱导上皮细胞永生化的机制研究 -- --万方医学网-医学杂志-医学期刊-医学论文-中华医学会期刊-中国医师协会期刊-医学外文文献 于本实验室-80℃冰箱保存。⑵收取黏膜上皮组织，Dispase酶及胰酶双重消化法制备上皮单细胞悬液，角化细胞无血清培养基(Keratinocyte-serum free medium，K-SFM)培养。免疫细胞化学(Immunocytochemistry，ICC)鉴定细胞的上皮来源。HaCaT细
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...取黏膜上皮组织，Dispase酶及胰酶双重消化法制备上皮单细胞悬液，角化细胞无血清培养基(Keratinocyte-serum free medium，K-SFM)培养。免疫细胞化学(Immunocytochemistry，ICC)鉴定细胞的上皮来源。
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在培养后期换用无血清培养基培养，细胞生长及蛋白表达水平正常。
During the later period of the cultivating process, the applying of serum free medium had no significant effects on the cell growth and protein expressing.
在这个无血清培养基中，SSV - NIH3T3细胞可以很好地生长，并具有分泌血小板生长因子样生物学活性物质和致裸鼠肿瘤的能力。
In this SFM SSV-NIH3T3 cells grow well, they keep the ability of secreting platelet-derived growth factor-like material into culture medium and causing tumor growth in nude mice.
方法从人胚胎海马区分离神经干细胞，采用无血清培养基，进行体外扩增培养、传代。
Methods The serum free culturing technology was used to isolate, culture and pass neural stem cells from embryonic human hippocampus.
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