骨髓造血细胞与浓缩病毒上清及细胞因子组合共培养。
The hematopoietic cells were cocultivated with concentrated virus supernatant and interleukins.
目的探讨不同细胞因子组合对脐带血ac133 +细胞体外扩增效率的影响。
Objective to investigate the effects of different cytokine combinations on the ex-vivo expansion of umbilical cord blood AC133 + cells.
方法在相同细胞因子组合下，用体外液体培养比较脐血有核细胞程序扩增组与一次扩增组及有无基质层条件下的优劣。
Methods under the same condition with hematopoietic factors in the liquid culture, compared the sequential expansion group and once for all expansion group with or without stroma cell layer.
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