...葡液,沈阳协和集团),CCK一8(武汉碧云天公司),异硫氰酸荧光素(FITC)标记的CD19/CD3/Cn4/CDS(美国BD公司),淋巴细胞分离液(天津TBD公司),RPMI一1640(Gibeo公司)。1.1.2肿瘤细胞系人胶质瘤U251细胞株.
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材料和方法一、材料hBMSCs(志愿者);Percoll细胞分离液(Pharmacia公司);胎牛血清(Hyclone公司);牛血清白蛋白(Sigma公司)。二、hBMSCs的分离与培养采集健康志愿者髂骨内骨髓15mL,加等.
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淋巴细胞分离液 Lymphocyte separation medium ; ficoll ; Ficoll-Paque Plus ; Ficoll-Hypaque Solution
小鼠细胞分离液 Mouse percoll
大鼠细胞分离液 Bandicoot percoll
人细胞分离液 Human cellular segregation
人淋巴细胞分离液 Lymphocytes Separation Medium
大鼠淋巴细胞分离液 Bandicoot percoll
小鼠淋巴细胞分离液 Mouse percoll
淋巴细胞分离液法 Method of lymphocyte apart fluid
使用淋巴细胞分离液和3%的明胶分离胎儿细胞。
Fetal cells were isolated using lymphocyte separation liquid and 3% gelatin.
利用淋巴细胞分离液分离出单核细胞，采用贴壁生长法分离纯化内皮祖细胞。
Lymphocyte separation medium was used to isolate the mononuclear cells, and the attachment growth method was used to purify endothelial progenitor cells.
方法：实验采用干细胞分离液，利用梯度密度法分离患者自体骨髓干细胞，制备干细胞悬液。
METHODS: Autologous BMSCs were isolated by density gradient centrifugation with stem cell extract liquid to prepare stem cell suspension, and were intramuscularly injected into the suffered limbs.
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