结论：在冻存液中加入10%DMSO和4%BSA，可在低温保存的过程中较好地保护细胞的存活及微囊的完整性。
Conclusion:The frozen stock solution with 10%DMSO and 4%BSA can preserve the cells and permeability of microcapsules in the process of low temperature preservation.
程序降温法：以甘油和二甲基亚砜为低温保护剂，分别以不同的比例添加到含有细胞悬液的冻存管中。
Programmed cooling method: The glycerol and Dimethylsulfoxide, as cryoprotective agents, were mixed with COC1 cells suspensions at different concentration, respectively.
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