肝组织GR采用放射性配体结合分析法测定其最大结合容量以及用免疫印迹法测定其蛋白含量。
Maximal binding volume of glucocorticoid receptor (GR) in hepatic tissue was assayed by radio-ligand binding assay and protein content was assayed by Western blot.
目的：确定蛋白免疫印迹检测大鼠脑组织中P-糖蛋白的最佳实验条件。
Objective: To determine the optimum experiment condition of detecting P—glycoprotein with Western blotting.
方法应用免疫组织化学方法检测erk在脊髓后角的定位表达，并用图像分析系统和免疫印迹法进行定量分析。
Methods The expression of ERK in spinal dorsal horn was detected by immunohistochemical method and was quantitated by western-blot analyses.
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