类人胶原蛋白(Human-like Collagen)是西安巨子生物基因技术公司 通过生物工程技术将人体胶原蛋白的mRNA逆转录成cDNA,经酶切后的一段基因重组于E.coli(大肠杆菌)内,经过高密度发酵、分离、复性、纯化工艺生产的一种高分子生物蛋白。主要的生产原料为葡萄糖和无机盐。由葡萄糖和无机盐仿制的胶原蛋白。
...类人胶原蛋白;纯化 [gap=1069]Key words: protein engineering; immobilized metal ion affinity chromatography; recombinant human-like collagen; purification ..
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The results showed that the internal model controller based on the neural network can be used in the process of recombinant E. coli in fed-batch culture to produce human-like collagen, and its proved to be fine in its robustness.
实验结果表明,基于神经网络的内模控制器可以用于类人胶原蛋白补料分批发酵过程,并具有良好的鲁棒性。
参考来源 - 重组菌生产类人胶原蛋白发酵过程建模与控制·2,447,543篇论文数据,部分数据来源于NoteExpress
目的建立重组类人胶原蛋白分离及纯化工艺。
Objective To develop a method for the purification of recombinant human collagen-like protein.
研究了应用金属螯合亲和层析法纯化重组类人胶原蛋白的条件。
Recombinant human-like collagen with a hexahistidine tail attacked to the carboxyl end could be easily purified by Metal Chelated Affinity Chromatography (MCAC).
结果表明:类人胶原蛋白膜和类人胶原蛋白海绵与组织相容性良好;
The results showed that HLC II film and HLC II sponge have good histocompatibility.
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