采用等位基因特异性引物(Allelic specific primer,ASP)PCR技术检测重庆地区汉族老年人群300例原发性骨质疏松症患者和93例对照者Klotho G-395...
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目的：研究建立等位基因特异性引物pcr技术体系，并将其应用于基因单核苷酸多态性研究工作。
Objective: to study and establish an allelic specific primer polymerase chain reaction (ASP-PCR) technique system and to apply this technique to study single nucleotide polymorphism (SNP) of genes.
目的：(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
Objective: (1) to establish PCR reaction system that USES allele-specific primer PCR technique to detect SNP of KLOTHO gene.
方法运用聚合酶链反应-序列特异性引物（PCR-SSP）法，对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。
Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong.
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