采用RT—PCR检测tankyrase1表达，端粒酶重复扩增法（telomerase repeat amplification protocol，TRAP）检测端粒酶活性，绘制生长曲线了解细胞生长状态，倒置显微镜观察和流式细胞仪检测细胞凋亡。
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采用端粒酶重复扩增法 telomericrepeatamplificationprotocol
端粒酶重复序列扩增法 Telomeric Repeat Amplifcation Protocol PCR ; TRAP-PCR ; telomeric repeat amplification protocol
方法取19例颅内肿瘤和5例脑外伤患者脑脊液标本，应用改良的银染端粒重复序列扩增法(TRAP)进行端粒酶活性检测。
Methods a modified telomeric repeat amplification protocal (TRAP) by silver staining was performed in 19 cases of intracranial tumors and 5 cases of brain injuries.
用端粒重复序列扩增法（TRAP）半定量方法测定细胞染毒前后端粒酶活性的变化；
The changes of telomerase activity after treatment was detected and quantified by telomeric repeat amplification protocol (TRAP) semi_quantitative analysis.
方法采用端粒重复序列扩增文件-酶标法（TRAP-ELISA）测定尖锐湿疣皮损中端粒酶的活性，并与正常皮肤和恶性肿瘤对照比较。
Methods The telomerase activity was detected by TRAP( telomeric repeat amplification protocol) -ELISA which based upon PCR amplification of the initial telomerase product and detected by ELISA.
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