...聚合酶链反应(polymerase chain reaction,PCR)技术建立的高敏感性端粒酶重复序列扩增(telomeric repeat amplification protocol,TRAP)检测技术已广泛用于恶性肿瘤的端粒酶活性研究,取得较好的效果。
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抗砷性的人骨髓间充质干细胞永生化及其分化的研究 -- --万方医学网-医学杂志-医学期刊-医学论文-中华医学会期刊-中国医师协会期刊-医学外文文献 名为hTERT-hBMSCs。应用RT-PCR检测hTERT在hTERT-hBMSCs细胞中的表达,端粒酶重复序列扩增(telomere repeatamplification protocol assay,TRAP)酶链免疫吸附试验(ELISA)法检测hBMSCs转染前后端粒酶活性的变化:用1μmol/L的亚砷酸钠
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端粒酶重复序列扩增法 Telomeric Repeat Amplifcation Protocol PCR ; TRAP-PCR ; telomeric repeat amplification protocol
方法采用端粒重复序列扩增文件-酶标法(TRAP-ELISA)测定尖锐湿疣皮损中端粒酶的活性,并与正常皮肤和恶性肿瘤对照比较。
Methods The telomerase activity was detected by TRAP( telomeric repeat amplification protocol) -ELISA which based upon PCR amplification of the initial telomerase product and detected by ELISA.
目的:建立端粒重复序列扩增(TRAP) -银染色技术,探讨端粒酶活性检测在肿瘤诊断中的意义。
Objectives: to establish a method of telomere repeat amplification protocol (TRAP) silver stain technique, and to investigate the significance of telomerase activity detection in tumor diagnosis.
用端粒重复序列扩增法(TRAP)半定量方法测定细胞染毒前后端粒酶活性的变化;
The changes of telomerase activity after treatment was detected and quantified by telomeric repeat amplification protocol (TRAP) semi_quantitative analysis.
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