目的:建立骨唾液蛋白的稳定表达乳腺癌细胞系,观察骨唾液蛋白在乳腺癌骨转移的整个过程中的作用。
OBJECTIVE: To establish breast cancer cell strains of BSP with stable expression and observe the effect of BSP in the whole process of breast cancer bone metastasis.
目的:构建人死亡受体5 (DR5)真核表达载体,转染NS - 1细胞,建立稳定转染的NS - 1细胞系。
Objective: to construct eukaryotic expression vector of human death receptor (DR5) and transfect NS-1 cells to establish stable NS-1 cell line.
目的:建立稳定表达EGFP标记的葡萄糖转运蛋白4的CHO细胞系,为研究GLUT4在CHO细胞中的转运调节机制奠定基础。
Objective:To establish a stable CHO cell line expressing GLUT4-EGFP, and to lay a foundation for investigating the translocation mechanisms of GLUT4 in CHO cells.
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