方法记录蟾蜍坐骨神经干动作电位和腓肠肌收缩曲线，测定低位运动中枢、神经-肌肉接头和骨骼肌出现疲劳的时间并加以比较。
Methods: the action potential of sciatic trunk and contract curve of sural muscle of hoptoad were recorded, and the time of fatigue in nerve center, neuro-muscular junction and muscle was checked.
脊椎动物的神经肌肉接头已经被很好地研究，很久以来人们认为乙酰胆碱是在这里被使用的唯一神经递质。
The vertebrate neuromuscular junction has been very well studied, and it has long been thought that acetylcholine was the only neurotransmitter used there.
持续长时间牵拉造成神经肌肉接头处的损害亦导致肌肉去神经支配。
Damage to the neuromuscular junction following prolonged retraction can also lead to muscle denervation.
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