方法采用碱性磷酸酶抗碱性磷酸酶法(APAAP法)检测48例急性荨麻疹患者和20例健康对照者外周血CD 3 +,CD 4 +,CD 8 + T淋巴细胞。
Methods the peripheral blood t lymphocytes and their subsets in 48 patients with acute urticaria were detected by APAAP and compared with 20 healthy control.
方法:用碱性磷酸酶抗碱性磷酸酶法检测再障患者骨髓中T淋巴细胞表型的变化,并用半固体培养法检测再障患者骨髓及去T细胞后骨髓单个核细胞CFU-GM集落形成情况。
Methods: Phenotype of T lymphocytes in bone marrow and CFU GM yields of bone marrow mononuclear cells with or without T cells were assayed with APAAP and semi solid culture method, respectively.
采用酶动力法测成骨细胞在基质材料上培养2,4,6 天后的碱性磷酸酶(ALP)活性。
Alkaline phosphatase (ALP) activity of osteoblasts was determined by enzyme dynamical method at 2d, 4d and 6d .
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