...基因表达的影响 Nell-1的bMSCs为试验组,用实时定量 PCR(real-time PCR)测定成骨相关基因 碱性磷酸酶基因( alkaline phosphatase, ALP)、骨涎蛋白(bone sialoprotein, BSP)、骨钙素(osteocalcin,OC)...
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...表达的影响 Nell-1的bMSCs为试验组,用实时定量 PCR(real-time PCR)测定成骨相关基因 碱性磷酸酶基因( alkaline phosphatase, ALP)、骨涎蛋白(bone sialoprotein, BSP)、骨钙素(osteocalcin,OC)...
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成骨诱导后检测碱性磷酸酶(ALP)活性，RT - P CR分析其成骨相关基因的表达情况。
Alkaline phosphatase (ALP) activity was assayed as well as to assess gene expression by RT-PCR.
结果转染后成骨细胞的基因组中整合有SV40T基因，碱性磷酸酶染色阳性细胞占95％以上；
Results Homologous integration of SV40T gene into RAE - 1 cell genome was positively detected by PCR. More than 95% cells were proved positively by alkaline phosphatase staining.
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