目的白藜芦醇对高糖诱导的近端小管上皮细胞(proximal tubule epithelial cells,PTEC)氧化应激的影响。方法采用手工微分离法体外培养大鼠PTEC,行 .
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目的探讨庆大霉素对实验性大鼠肾近端小管上皮细胞凋亡的影响。
Objective to discuss the effects of gentamicin nephrotoxicity on the apoptosis of proximal tubule epithelial cells of rats.
方法:1、应用MTT方法在细胞水平上进行中药粗提物的筛选,首先建立人肾脏 近 端 小 管上皮细胞株(HK-2)及卵巢癌细胞株(OC-3)的生长曲线,以确定接种密度及给药时机;
Methods: 1. In vitro, with MTT, the growth curve of HK-2 cells and OC-3 cells had been built in order to decide the density to inoculate and when to administration.
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