...应用一系列已知抗HLA的特异性标准 分型血清与待测淋巴细胞混合,借助补体介导 的细胞毒试验(complement dependent cytotoxicity,CDC)。
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补体介导的细胞毒试验 Complement Mediated Cytotoxicity Test
补体依赖的细胞毒试验 complement dependent cytotoxicity test ; CDC
方法采用MTT法进行NK细胞的细胞毒试验和用流式细胞仪测定淋巴细胞CD抗原表达。
Method the cytotoxicity of NK was measured by MTT assay and the express of CD antigen on lymphocytes examined by FACS.
结论三次项曲线对MTS法细胞毒试验的细胞数od关系有很高拟合度且优于线性曲线模型。
CONCLUSION For the relations of cell number-OD in MTS cytotoxicity assay, the fitness of cubic curves are better than the linear model.
方法:采用国际通用的微量淋巴细胞毒试验和混合淋巴细胞培养方法;
Methods:The conventional microlymphocytotoxicity test and the lymphocyte co cultivation were used.
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