方法 采用以PCR为基础结合酶联免疫吸附试验（ELISA）的端粒重复序列扩增（Telmoerase repeat amplification protocol,TRAP）法对35例患者体腔液沉淀细胞端粒酶活性进行相对性定量测定，并与脱落细胞常规病理学检查结果进行平行比较...
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方法采用端粒重复序列扩增文件-酶标法（TRAP-ELISA）测定尖锐湿疣皮损中端粒酶的活性，并与正常皮肤和恶性肿瘤对照比较。
Methods The telomerase activity was detected by TRAP( telomeric repeat amplification protocol) -ELISA which based upon PCR amplification of the initial telomerase product and detected by ELISA.
用端粒重复序列扩增法（TRAP）半定量方法测定细胞染毒前后端粒酶活性的变化；
The changes of telomerase activity after treatment was detected and quantified by telomeric repeat amplification protocol (TRAP) semi_quantitative analysis.
目的：建立端粒重复序列扩增(TRAP) -银染色技术，探讨端粒酶活性检测在肿瘤诊断中的意义。
Objectives: to establish a method of telomere repeat amplification protocol (TRAP) silver stain technique, and to investigate the significance of telomerase activity detection in tumor diagnosis.
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