结直肠癌细胞系肿瘤干细胞相关亚群初步研究 -- 重庆医科大学学报 --万方医学网-医学杂志-医学期刊-医学论文-中华医学会期刊-中国医师协会期刊-医学外文文献 癌细胞系LOVO、SW480、HCT116中的侧群细胞(Side population,SP)细胞,添加生长因子的无血清培养基(Serum-free medium,SFM)培养SW480初步分离,富集肿瘤干细胞样亚群,有限稀释实验,诱导分化,传代更新,含血清/无血清培养基
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方法 在添加生长因子的无血清培养基(serum free media, SFM)中悬浮培养细胞获得富含BCSCs的微球体(mammospheres, MSs),通过克隆形成实验研究...
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的无血清神经培养基 neurobal medium
本发明提供了一种用于大规模培养CHO细胞的无血清培养基。
The present invention provides a serum free medium used for cultivating CHO cellular extensively, and discloses the prescription of the culture medium.
该培养基与商品化的无血清培养基比较,能够使细胞生长维持较长的时间,表达产物分泌量也相对较高。
In comparison with commercial SFM, it can maintain growth of CHO cell for much longer peroid and the expressing level of HBsAg is higher too.
通用的昆虫细胞培养基配方的不断优化,特定昆虫细胞株培养基的研制和适用于大规模悬浮培养的无血清培养基的商品化;
Instect cell culture media were optimized carefully to find new medium for special cell strain and serum free media was manufactured commercially for suspension cell mass production;
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