...文 rn blot法分析这些细胞系及野生型和IKKβ基因敲除(IKKβknockout，IKKβ-／-)的小鼠胚胎成纤维细胞(mouse embryo fibroblast，MEF)中p21的总蛋白水平，并对上述稳定细胞系作胞浆胞核分离，比较不同细胞组分中的p21蛋白水平。
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小鼠的胚胎成纤维细胞 mouse embryonic fibroblast
目的探讨建立有效的小鼠胚胎成纤维细胞饲养层，用于胚胎干细胞的培养。
Objective To study the isolation and culture and application of Kunming mouse embryonic fibroblasts(MEF).
目的 在本实验室建立小鼠胚胎成纤维细胞培养方法，并在此基础上用培养的细胞检测氯化镉、甲醛等外来化合物的细胞毒性反应。
Objectives: To set up the methods of mouse embryo fibroblasts culture in vitro and to evaluate the toxicity of xenobiotics such as chloride cadmium and formaldehyde by using these methods.
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