利用淘汰奶牛卵巢生产体外受精胚胎 In Vitro Production of Cattle Embryos Derived from Abattoir Ovaries-作者:张德福,王英,张忠明,韩增生,陈晓羽,吕俊,张军民,徐直 .5%停留在 8～ 16-细胞期,最高发育阶段为 16-细胞期;有 3层以上卵丘细胞紧密包围的卵丘-卵母细胞复合体( Cumulus- Oocyte Complex,COC)经成熟培养和体外受精后,卵裂率( 64.5%VS 54.7%, p<0.01)和囊胚发育率( 34.
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...留在 8～ 16-细胞期,最高发育阶段为 16-细胞期;有 3层以上卵丘细胞紧密包围的卵丘-卵母细胞复合体( Cumulus- Oocyte Complex,COC)经成熟培养和体外受精后,卵裂率( 64.5% VS 54.7% , p<0.01)和囊胚发育率( 3...
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分别用小鼠、大鼠、猪的卵丘细胞与小鼠pno和NO进行了共培养。
Mouse PNO and NO were cultured with cumulus cells isolated from mouse, rat and swine, respectively.
电镜下观察可见：甲醛的中、高剂量组卵母细胞崩解液化，周边的卵丘细胞内线粒体肿胀及空泡变；
The mitochondria of oocytes and cumulus cells in high and medial dose groups swelling and vacuolar degeneration, but rough surfaced endoplasmic reticulum (RER) were normal and abundance.
卵丘细胞对卵母细胞内的谷胱甘肽合成起重要作用。
Cumulus cells play an important role in the intracellular GSH synthesis in oocyte.
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