本实验室利用基因重组技术构建了抗鼻咽癌单克隆抗体BAC5的单链抗体(single-chainFvantibodyfragement，ScFv)的原核表达载体，并转入大肠杆菌中进行诱导表达，得到了以包涵体形式表达的无活性的BAC5-ScFv。
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特异性结合的单链抗体 single-chain Fv ; SCFV
凝血酶特异的单链抗体 Thrombin-specific scAb
的特异性单链抗体 single chain fragment variable ; SCFV
蛋白的人源性单链抗体 single chain Fv fragment ; SCFV
对复性后的单链抗体以抗原捕获ELISA法进行检测。
The refolded single-chain antibodies were examined by antigen capture ELISA.
为获取识别多种WSSV抗原的抗体提供了新的方法路线， 也为获取特异性识别线性表位的单链抗体提供了一种新的淘选技巧。
This study provides a new way to obtain more different antibodies specifically binding to WSSV, and especially provides a new strategy to obtain scFvs against linear epitopes.
目的探讨在不同的免疫学检测方法中，与单链抗体相连的亲和标记肽对单链抗体与抗原结合活性的影响。
Objective to investigate the affects of affinity tag on binding activity of single chain variable fragment (ScFv) to antigen using different immunological assays.
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