组织水平：在各观察点通过检测脑组织中伊文氏蓝(EB)的含量，定量分析安定与氟马西尼对BBB通透性的影响。

Tissue level: Examining the content of the Evan's blue (EB) inside brain tissue was used to analysis the effect of diazepam and flumazenil on the BBB permeability quantitatively.

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采用荧光显微镜伊文氏蓝(EB)、电镜镧示踪法以及透射电镜观察脑组织的超微结构变化。

To observe the change of brain tissue and ultrastructure by fluorescence microscope with Evans Blue (EB) dyeing, electro-microscope with La footprint and transmission electro-microscope.

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