前言：目的：比较不同的人牙髓细胞原代培养方法，优化培养条件，提高培养成功率。
AIM: to compare the primary culture methods of human pulp cells, to enhance the culture condition, and give rise of the success rate of cultivation.
方法：采用经具核梭杆菌LPS刺激的单核细胞培养上清作用于体外培养的人牙髓细胞，通过OD值测定，观察单核细胞培养上清对人牙髓成纤维细胞碱性磷酸酶（ALP）活性的影响。
Methods:The culture supernatant of monocytes stimulated with Fn LPS was applied to human dental pulp cell in vitro, and activity of ALP in human dental pulp fibroblast was monitored by OD test.
目的研究第三恒磨牙来源的人牙髓干细胞的表型和生物学性状。
Objective To isolate and identify human dental pulp stem cells from third molars.
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