...腔网 法观察了在正常和经骨形成蛋白(bone morphogenetic protein,BMP)作用后,离体培养的人牙周膜细胞(periodontal ligament cell,PDLC)的DNA合成情况和细胞周期变化,探讨影响其增殖的原因和意义。
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...法观察了在正常和经骨形成蛋白(bone morphogenetic protein,BMP)作用后,离体培养的人牙周膜细胞(periodontal ligament cell,PDLC)的DNA合成情况和细胞周期变化,探讨影响其增殖的原因和意义。
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的人牙周膜成纤维细胞 human periodontal ligament fibroblast
方法：通过原代和传代培养，获得性状稳定的人牙周膜细胞，使用动态机械应变细胞加载仪对细胞进行1%、10%和20%动态牵张应变加载。
METHODS: Primary human periodontal ligament cells were obtained and passaged. Then the cells were stretched by dynamic mechanical strain unit with 1%, 10% and 20% strain at different time.
结果：加载模拟咬合压力的人牙周膜成纤维细胞COX 2表达水平明显高于对照组，而且在一定范围内表达水平与加压力值成剂量依赖关系；
Res ults: COX-2 expression level in HPLFs loaded with intermittent simulated occlusion pressure was significantly higher than that in the controls in a magnitude-dependent manner.
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