...建肝细胞永生化载体过程中,为了避免t抗原的表达,同时缩短DNA长度,采用重叠延伸拼接法(splicingby overlapping extension,SOE)法[14-15]将SV40T的2个外显子拼接起来,去除了内含子,并将重组的SV40T克隆到逆转录病毒...
基于2个网页-相关网页
方法采用重叠延伸拼接法(splicing by overlap extention, SOE)将anti-CD3 scFv和B7.1(V+..
基于2个网页-相关网页
采用重叠延伸拼接法(splicing by overlapping extension, SOE) 将轻重链基因连接成单链基因, 具体过程如下: 首先将纯化后的轻重链基因混匀, 在无外加引物的...
基于2个网页-相关网页
采用重叠延伸拼接法 splicing by overlapping extension ; splicing by overlap extention
应用推荐