【用重叠延伸拼接】

用重叠延伸拼接

splicing by overlap extension

...瘤体外构建U6/H1双启动子SECs及其对HepG2细胞端粒酶活性抑制的研究目的：利用重叠延伸拼接(splicing by overlap extension，SOE)PCR技术构建相对排列的U6/H1双启动子siRNA表达框架(siRNAexpression cas...

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SOE

...体外构建U6/H1双启动子SECs及其对HepG2细胞端粒酶活性抑制的研究目的：利用重叠延伸拼接(splicing by overlap extension，SOE)PCR技术构建相对排列的U6/H1双启动子siRNA表达框架(siRNAexpression cas...

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短语

采用重叠延伸拼接法 splicing by overlapping extension ; splicing by overlap extention

利用重叠延伸拼接 splicing by overlap extension ; SOE

用重叠延伸拼接法 SOE ; splicing by overlap extention ; splicing by overlapping extension

用重叠延伸拼接

Use overlapping extension splicing

以上为机器翻译结果，长、整句建议使用人工翻译。

方法用PCR方法扩增il_3信号肽序列和人内皮抑素基因，然后采用重叠延伸pcr拼接的方法将两者拼接和扩增。

METHODS IL_3 signal sequence and human endostatin gene were amplified with PCR and spliced with overlap extension PCR.

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