有文献报道在非变性的聚丙烯酰胺中加入甘油可提高SSCP检测的灵敏度。
It was reported that glycerol in the non-denatured SSCP polyacrylamide gel could increase the sensibility of detecting mutation.
同时,我们发现其它的添加物,如DMSO,甘油,甲酰胺对长片段pcr的作用不明显。
In the meantime we tried other additive chemicals, such as DMSO, glycerin, formamide. They were no notable results in long PCR.
以n, N -二甲基甲酰胺为溶剂合成单硬脂酸甘油酯。
Glycerin monostearate was prepared by using n, N-dimethylformamide (DMF) as solvent.
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