目的：比较载杆玻璃化冷冻法与程序冷冻法对人早期胚胎冷冻复苏的效果。

Objective To compare the outcome of the vitrification using cryotop to the slow-freezing method for human cleaved embryos cryopreservation.

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以常规的慢速冷冻法为对照，研究了玻璃化冷冻法保存绵羊体外受精囊胚的效果。

Then, the PGCs were froze respectively in six kinds of freezing medias under slow speed cryopreservation process and vitrification process.

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用细管法玻璃化冷冻和两步解冻法可显著提高体外受精胚胎的存活和生长。

The rates of survival and usability of vitrified by straw method and two-step thawing method could be improved significantly.

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