方法：采用PCR，克隆及连续亚克隆，序列分析，原核温度诱导表达等方法。
Methods: PCR, gene cloning and successive sub cloning, DNA sequencing, prokaryotic temperature induction, etc. were used.
融合蛋白表达量的高低与诱导时的细胞密度，诱导的温度以及培养基有关。在一定范围内与诱导剂的剂量以及诱导时间的长短无关。
The expression level was closely related with the cell density, induction temperature and medium but not with the inductor dosage and the induction period within certain range.
通过对表达载体的SDS-PAGE 各种参数包括时间、温度和IPTG 浓度诱导表达分析，结果显示最佳诱导时间为3～4 小时；
Through induced expressing and analyzing on various kinds of SDS-PAGE parameter including time, temperature and IPTG density, the result showed that the best induced time was 3~4 hours;
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