...淋巴细胞混合培养； 一氧化氮； 细胞因子； 免疫耐受 [gap=1874]Key words】 Liver transplantation; Kupffer cell; Mixed lymphocyte culture; Immunotolerance; Nitric oxide; Cytokine ..
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方法：在体外，三种肿瘤细胞与同上鼠脾淋巴细胞混合培养（MLTCS）后，测定淋巴细胞增殖指数（MTT法）和特异杀伤活性（LDH释放改良法）；MTTI地测定肿瘤细胞体外增殖能力后，将B16-neo和B16-mB7-1...
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...究 取正常人的外周血单个核细胞(PeripheralBloodMononuclearCell，PBMC)进行单向淋巴细胞混合培养(MixedLymphocyteCulture，MLC)5d后，分别加入终浓度为0.5mmol/L、lmmol/L、1.5mmol/L、2mmol/L、2.
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淋巴细胞进行混合培养 mixed lymphocyte culture ; MLC
MTT法测定混合淋巴细胞培养反应。
以供者及无关者脾细胞为刺激细胞，受者淋巴细胞为反应细胞，做混合淋巴细胞培养。
In the mixed lymphocyte culture, donor and third-party's spleen cells were stimulation cells, recipient's lymphocytes were reaction cells.
目的：观察在体外混合培养条件下，抗原能否诱导外周淋巴细胞反应抑制，建立体外淋巴细胞反应抑制模型。
AIM: To observe whether antigens can induce the restrained peripheral lymphocytes response under mixed culture in vitro, and establish a model of restrained lymphocytes response in vitro.
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