方法应用淋巴细胞杂交瘤技术，制备单克隆抗体，并对其进行鉴定。
Methods McAb were prepared and identified by applying the lymphocyte hybridoma technique.
建立杂交瘤单抗亲和层析纯化抗原、抗原体外致敏淋巴细胞和RT-PCR克隆人抗体基因及噬菌体呈现技术构建人源抗体库的策略。
Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology.
作者采用小鼠骨髓瘤细胞系与破伤风类毒素免疫的人外周血淋巴细胞进行融合，对影响人-鼠杂交瘤细胞制备人单抗成功的因素进行了探讨。
The cells from mouse myeloma cell line SP2/0 were fused with human peripheral blood lymphocytes from the donors primed with a conventional dosage of tetanus toxoid (TT).
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