测定体重、肺湿重;测定肺组织羟脯氨酸（Hydroxyproline,HP）;病理形态学检查,HE染色、vG染色;免疫组织化学染色法观察各组实验动物肺组织Ⅰ、Ⅲ型胶原蛋白的表达...
基于4个网页-相关网页
将这一方法与肺组织羟脯氨酸含量测定相比较，表明二者在显示肺纤维化程度和变化趋势上具有相同的结果。
Compared with lung hydroxyproline content measurement, it was shown that both have a similar result in displaying the extent of pulmonary fibrosis and its alteration trend.
采用分光光度法测定肺组织匀浆羟脯氨酸含量。
Hydroxyproline was measured in lung tissue homogenates by spectrophotometer.
方法：羟脯氨酸法、间接酶联免疫吸附法（ELISA）测定博莱霉素A6（BLMA6）诱发的小鼠、大鼠肺纤维化模型肺组织胶原含量及合成，阿利新蓝法测定其糖胺多糖（GAG）含量；
METHODS: Hydroproline determination and ELISA were used for measuring the content of collagen, Alcian blue assay for BLM-A6 induced glycosaminoglycan (GAG) in the lung tissues (rats, mice).
应用推荐