利用磁吸附细胞分选法(MACS)分离大鼠脾脏CD 4 + T细胞，流式细胞术检测MACS分选前后CD 4 + T细胞比例，监测细胞分选效果。

Magnetic absorption cell sorting (MACS) method was used to isolate the CD4 + t lymphocytes from the spleen of the rats. Flow cytometry was used to monitor the efficiency of isolation.

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结果①流式细胞术分选后共聚焦显微镜分析技术（PSC）能同时、准确地鉴别不同细胞周期时相的凋亡细胞和非凋亡细胞。

Results Post-sorting confocal (PSC) method could exactly distinguish apoptotic and non-apoptotic cells in the specific cell cycle phases at the same time.

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