进化酶基因的DNA测序表明4 186含两个有义氨基酸置换：k16 7r和S374C，二者既不位于底物结合位点，也不位于酶的金属离子结合位点。
DNA sequence revealed that 4-186 contains two amino acid substitutions, K167R and S374C, both of which locate neither the substrate - binding sites nor the metal - binding sites of EAP.
单一氨基酸的置换可以改变酶的热稳定性。
Thermostability of enzymes can be altered by single amino acid substitution.
这两个点变异，将导致两个氨基酸的置换(Y154H、E348K)。
These substitution results in changes in two amino acid residues (Y154H and E348K).
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