The results showed here indicate that spermine can specially stimulate the aminoacylation of bovine liver tRNA~(Ile), and markedly increase the Vmax value of the reaction and the K_m value for tRNA~(Ile), but cause no change in IleRS activity.
结果表明:精胺能特异地促使牛肝tRNA~(Ile)氨基酰化反应;对IleRS活性无影响;能明显地增加形成Ile-tRNA复合物反应的Vmax和tRNA~(Ile)的Km值。
参考来源 - 精胺对牛肝tRNA~(lle)氨基酰化的刺激作用·2,447,543篇论文数据，部分数据来源于NoteExpress
以Y型分子筛为载体，分别采用吸附法、吸附-戊二醛交联法、偶联法和偶联-重氮法4种方法对氨基酰化酶i进行固定化。
Acylase I was immobilized on Y-type molecular sieve by using 4 kinds of methods, namely, adsorption, adsorption-crosslink, coupling and coupling-diazotization.
最后，科学家将剩余的氨基进行乙酰化，这保证了复杂粒子不会受到表面电荷影响。
Finally, the remaining amine groups are acetylated to ensure that the complex particles do not bear any surface charges.
对一些物质，如d, L丹酰化氨基酸的拆分有一定的规律，能给出绝对构型信息。
The resolution of some chiral drugs such as d, L-dansyl amino acid has certain rule, and provides information about absolute configuration.
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