此类溶酶体中含有水解酶和相应的底物，是一种将要或正在进行消化作用的溶酶体。根据所消化的物质来源不同， 分为自噬性溶酶体、异噬性溶酶体。 次级溶酶体（secondarylysosome）是含水解酶及相应底物，以及水解消化的产物，也即正在进行或已经进行消化作用的囊泡。所以，次级溶酶体又称活动性溶酶体（active lysosome）或消化泡（digestionvacuole）。其分布较广，形态具有多样性。
肺泡巨噬细胞次级溶酶体明显增多。
In alveolar macrophage the increasing secondary lysosomes are seen.
次级溶酶体和小颗粒呈MPO强阳性。
The secondary lysosomes and the small granules are strongly positive to MPO.
表明缺硼引起溶酶体的发育，由小液泡的原初溶酶体变成了大液泡的次级溶酶体。
Dark fibrilar substance appeared in the vacuole. It indicated that the lysosomes were caused by boron deficiency from pro-lysosome of small vacuole developed into secondary ones of big vacuole.
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