...后对肿瘤标本进行病理组织学检查,测定肿瘤坏死面积,检测原位末端标记(TdT-mediated dUTP nick end labeling technique,TUNEL)及增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)。
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分别采用免疫组化方法和原位末端标记法检测垂体前叶细胞增殖核抗原的表达及凋亡的变化情况。
The changes of the proliferating nuclear antigen expression and apoptosis of anterior pituitary cells were detected with immunohistochemistry and in situ end labeling method.
观察各组家兔血清心肌酶学变化,并通过电镜形态学及原位末端标记法检测心肌细胞凋亡情况。
Then we observe the change of myocardium enzymatic dynamics and detect myocyte apoptosis with electronic microscope and situ end labeling way.
方法采用原位切口末端标记方法检测细胞凋亡,平均每100个细胞内的阳性细胞数为凋亡指数(AI)。
Method Apoptosis was determined by the method of in situ Nick end labeling. Apoptotic index (AI) means the Numbers of apoptotic cells per 100 cells.
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