根癌农杆菌介导转化 Agrobacterium tumefaciens-mediated transformation ; ATMT
根癌农杆菌介导的转化 Agrobacterium tumefaciens-mediated transformation
MethodWith callus of Kasalath as transformation receptor,Agrobacterium tumefaciens-mediated method was used to conduct genetic transformation. The genetic transformation system was optimized from several aspects,including co-culture mode,coculture time and the aftertreatment method of co-culture.
方法以籼稻品种Kasalath的愈伤组织为转化受体,应用根癌农杆菌介导法进行遗传转化;从共培养方式、共培养时间、共培养后处理方法等方面优化遗传转化体系。
参考来源 - 籼稻品种Kasalath遗传转化条件的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
通过对影响根癌农杆菌介导蓝猪耳转化效果各种因素的研究，建立了蓝猪耳高效稳定的遗传转化系统。
An efficient and stable Agrobacterium-mediated genetic transformation system for Torenia was established through investigating the factors influencing the transformation efficiency.
采用根癌农杆菌介导法将油菜CBF1基因转入拟南芥，筛选抗性转基因拟南芥植株，进行PCR检测和GUS染色。
CBF1 gene from rape was transformed into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated method to screen its transgenic plants with resistance and conduct PCR detection and GUS staining.
目前用于油菜基因转化的方法有根癌农杆菌介导法、基因枪法、PEG法、激光微束穿刺法、显微注射法和花粉介导法等。
At present, there are several methods of transformation in rape, including tumefaciens-medidated genetic etc, particle gun, PEG untake, vialaser microbeam puncture and microinjection.
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