摘要01 关键词： 猪生殖与呼吸综合征病毒；ORF3基因；ORF5基因；克隆；杆状病毒表达载体[gap=1663]Key words: PRRSV;ORF3 gene;ORF5 gene;cloning;baculovirus expression vector
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杆状病毒载体表达系统 BEVS ; baculovirus expression vector systems
目的利用杆状病毒表达载体系统制备重组乙型肝炎病毒核心蛋白。
Objective To produce recombinant HBcAg in insect cells by Baculovirus expression vector system.
另外，杆状病毒是非复制型载体，能高效表达目的蛋白，其作为基因转移载体在基因治疗中乙显示出良好的应用前景。
Additionally, baculovirus has been a new gene transfer vector used in gene therapy due to its advantages of the extremely high expression of foreign genes and being non-reproduce vector.
结论：杆状病毒载体能够介导HHV - 8肿瘤转化基因k12编码的蛋白在昆虫细胞中表达。
Conclusion: Baculovirus transfer vector may mediate the coding protein of HHV-8 K12 gene expressed in insect cells.
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