...肿瘤抗侵袭转移治疗研究的基本策略和思路,通过基因工程及蛋白质工程,把与uPAR结合的uPA氨基末端片段(amino-terminal fragment, ATF) 的cDNA通过含十一个氨基酸残基的连接肽编码序列与缺失了CD螺旋间区第3398位氨基酸残基的、不再...
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...摘】目的观察曲美他嗪对老年代谢综合征(MS)患者血浆脑钠肽前体裂解的N-末端片段(N-terminal pro-B-type natriuretic peptide,N-proBNP)浓度的影响,探讨其对MS患者心脏舒张功能的保护作用。方法选择符合条件者进入研究。
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...察曲美他嗪对老年代谢综合征(MS)患者血浆脑钠肽前体裂解的N-末端片段(N-terminal pro-B-type natriuretic peptide,N-proBNP)浓度的影响,探讨其对MS患者心脏舒张功能的保护作用。方法选择符合条件者进入研究。
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为了解人类LDL受体基因内含子15的遗传背景,利用长链PCR和锚定PCR分离了LDL受体基因外显子15-内含子15-外显子16和内含于15的3’末端片段。
To understand intron 15 of human LDL receptor gene, the DNA fragments from exon 15 to exon 16 and the 3' end of intron 15 were amplified with long chain PCR and anchored PCR.
在每一个片段的末端,研究者连上一个荧光蛋白,在另一端他们工程DNA能熄灭荧光的亮度。
At one end of each snippet, the researchers attached a fluorescent protein, and, at the other end, they engineered the DNA so that it could "quench" the fluorescent light.
为了更好地处理低质量片段,BOAT支持一个“3'末端修剪模式”以构建测序片段局部优化的比对,进一步提高灵敏度。
For better handling of low-quality reads, BOAT supports a "3'-end Trimming Mode" to build local optimized alignment for sequencing reads, further improving sensitivity.
If you cut and you open up a segment of DNA then you've left these sticky ends, for example and these sticky ends are capable of recognizing each other by the process of hybridization.
如果你进行切割打开一个DNA片段,就会留下,比如说,这些粘性末端,这些粘性末端能在杂交的过程中互相识别
Now if I put them in contact with one another, the plasmid that's been opened and fragments of the DNA - special fragments that I've produced with the same restriction enzyme, they'll have the same sticky ends, they will naturally hybridize with one another.
如果我让它们互相接触,已经切开的质粒和DNA片段--,这DNA片段是我已用同种限制性内切酶,处理过的特别片段,这两者会有相同的粘性末端,它们会自然地相互杂交
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