方法免疫组织化学染色结合显微图像定量分析和免疫荧光双重染色。
Methods Immunohistochemical staining combined with the micro-image analysis and immunofluorescence histochemical double-staining technique were used.
免疫组织化学染色片显微镜下放大200倍，每个标本取6张切片，随机取5个视野，计算免疫组织化学染色的平均吸光度值和积分吸光度值进行半定量分析。
Under the microscope magnifying 200,6 sections of each sample in 5 random sights were selected to conduct the semi-quantitative analysis of the mean absorbance (Am) and integral absorbance (Ai).
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