...无血清悬浮培养 乳腺癌4T1细胞 肿瘤干细胞 CD44^+CD24^-/low 侧群细胞[gap=1970]Key words: serum-free suspension culture; breast cancer 4T1 cell; cancer stem cell; CD44^+CD24^-/low; side population ce..
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方法利用无血清悬浮培养、单细胞克隆技术分离神经上皮干细胞；
Methods Serum free cells suspension culture and single cell cloning technique were used to isolate neuroepithelial stem cells.
通用的昆虫细胞培养基配方的不断优化，特定昆虫细胞株培养基的研制和适用于大规模悬浮培养的无血清培养基的商品化；
Instect cell culture media were optimized carefully to find new medium for special cell strain and serum free media was manufactured commercially for suspension cell mass production;
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