脾脏放置在含有细胞培养用的20毫升10%胎牛血清培养皿里，剪成两瓣，用无菌玻璃的边缘挤压，获得单细胞悬液。
Place the spleen in a Petri plate with 20 ml of RPMI 10% FBS, cut in two pieces and squeeze each of them between two sterile glass slides to obtain a single cell suspension.
各膜来单独包装在无菌细胞培养皿。
Each membrane comes individually packaged in a sterile cell culture dish.
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