张海伶,郭文有,路荣海,张信,大肠癌中MMP-2、TIMP-2基因的表达及其对预后的影响 言 目的研究大肠癌中MMP-2、TIMP-2基因的表达,旨在探讨其在大肠癌局部进展中的作用。方法应用流式细胞术(flowcytometrytechnique,FCM)测定60例大肠癌和15例正常对照组结肠标本的MMP-2、TIMP-2基因蛋白表达量,蛋白表达量以荧光
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方法应用流式细胞术和细胞免疫荧光染色技术。
Methods It was studied by flow cytometry and immunofluorescence.
方法应用流式细胞术分析人胃癌MGC803细胞在DADS处理前后细胞周期分布的变化。
Methods cell cycle of MGC803 cell treated with DADS in vitro was analyzed by flow cytometry.
方法应用流式细胞术检测16例正常人和32例肾癌患者外周血淋巴细胞CD25、Ki67的表达。
Methods Peripheral blood lymphocytes CD25 and Ki67 were detected by flowcytometry in 32 patients with RCC and 16 healthy individuals served as controls.
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