通过硝酸纤维膜斑点印迹法进行阳性克隆鉴定。
Sandwich ELISA, dot blotting and DNA sequencing methods were used for the identification of positive clones.
采用斑点印迹法观察黑色普氏菌能否与人血红蛋白结合。
Dot blot assay was adopted to observe the binding of hemoglobin by P. nigrescens.
经生物素标记探针杂交确认RANTES基因的整合，用免疫斑点印迹法对重组基因表达水平进行定量测定。
The integration of RANTES gene was confirmed by hybridization with biotin-labelled probes and the recombinant gene expression level was quantitatively detected by immunodot blotting.
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