...癌细胞株应用去甲基化剂5-Aza-CdR诱导处理,结 合甲基化敏感性聚合酶链反应(Methylation Sensitive Polymerase Chain Reaction,MSP)方法,探讨胃癌细胞中RASSFlA基因异常机制。
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目的探讨荧光定量聚合酶链反应(FQ PCR)与常规聚合酶链反应检测乙型肝炎病毒(HBV)敏感性的差异。
Objective To explore the sensitivity difference between fluorescent quantity PCR (FQ-PCR) and routine PCR for HBV DNA detection.
目的探讨单管巢式聚合酶链反应(SNPCR)检测石蜡包埋组织结核分支杆菌dna的特异性和敏感性。
Objective To study the sensitivity and specificity of single tube nested polymerase chain reaction (SN PCR) technique in detecting Mycobacterium tuberculosis DNA in paraffin embedded tissues.
方法采用微量稀释法检测21种抗菌药物的敏感性,运用聚合酶链反应(PCR)技术检测氨基糖苷类修饰酶基因。
METHODS The dilution test was performed to detect the susceptibility to 21 kinds of antimicrobial agents and genotypes of AMEs of 20 E. coli strains were analyzed by polymerase chain reaction(PCR).
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